metaSPAdes Assembly =================== SPAdes – St. Petersburg genome assembler – is an assembly toolkit containing various assembly pipelines. See the `SPAdes home page `_ for more info. metaSPAdes can be run by the following command:: cd /mnt/WGS-data metaspades.py -o metaspades_out --pe1-1 read1.fq --pe1-2 read2.fq The contig sequences are located in the `metaspades_out` directory in file `contigs.fasta`. Again, let's get some basic statistics on the contigs:: getN50.pl -s 500 -f metaspades_out/contigs.fasta